3 speciﬁc antibody was purchased from Abcam (Ab13847) to detect the cleaved 17 kD form of caspase-3 by Western blotting. Protein AG agarose was purchased from Pierce (Waltham, MA, USA). HEK293 (RRID:CVCL_0045) and 769-P (RRID:CVCL_1050) kidney cells lines were sourced originally from ATCC and respectively maintained in DMEM

dilution (caspase3, ab13847; caspase9, ab32539; p65, ab16502; IKKα, ab178870; IκBα, ab109300; Abcam, Cambridge, MA, USA) overnight. After washing the cells with PBS, we incubated the cells with fluorescent secondary antibody dilution (Sigma-Aldrich, St. Louis, MO, USA) for 1 hour in the dark. We then washed the cells with PBS

(ab13847) Anti-Caspase-3 antibody Antibody info; Additional info; Supplier Abcam. Host Rabbit Type Primary Clonality Polyclonal Conjugate Unconjugated Target Caspase-3 UniProt P42574 - CASP3_HUMAN. Gene CASP3 Modification Unmodified View Product On Supplier's Website Request a Quote from Abcam. Supplier suggested.

Ab13847

UniProt Code History. A8K5M2, D3DP53, P42574, Q96AN1 Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199. Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA-1; Clonality Polyclonal; Validation KnockOut Validated The Ab13847) Anti-Caspase-3 Antibody Zebrafish reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact zebrafish Antibody.

Scientific. Rabbit anti-mouse Ki-67 ab15580.

ab13847. AB_443014. active Caspase 3 antibody Go To Vendor. active Caspase 3 antibody drosophila/arthropod, other mammalian, zebrafish/fish, mouse, rat,

Skriv ett omdöme. Rabbit anti-active caspase 3 antibodies, Abcam, ab13847. Normal goat serum, Vector Laboratories, S-1000.

ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. Invitrogen Anti-Caspase 9 (Cleaved Asp315) Polyclonal, Catalog # PA5-17913. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunoprecipitation (IP) applications. After blocked with blocking buffer (P0260, Beyotime, China) for 10 min at room temperature, the slices were incubated with antibodies against cleaved-caspase-3 (ab13847, Abcam, USA), cleaved active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 Anti-caspase3 Abcam ab13847 WB Anti-eIF2a Abcam ab5369 WB Anti-Sigma1 Santa Cruz Sc22948 IF Anti-CD3 (Alexa Fluor® 647-conjugated) BioLegend 100209 IF Anti-CD86 (APC-conjugated) BioLegend 105011 FC Anti-CD11b (Alexa Fluor® 488-conjugated) BioLegend 101217 FC Anti-CD11c (FITC-conjugated) BioLegend 117305 FC Anti-CD4 (FITC-conjugated) BioLegend Slides were stained to detect the expression of PRDX1 (abcam; Anti-PRDX1 antibody ab41906), γ-H2A.X (abcam; Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (abcam; Anti Ki67 antibody; ab 15580), 53BP1 (abcam; Anti-53BP1 antibody; ab21083) and cleaved caspase 3 (abcam; Anti-cleaved caspase 3 antibody; ab13847) using an HRP/DAB Equal amounts of proteins were separated by SDS-PAGE and transferred to a polyvinylidene fluoride membrane. After blocking for 1 h with 5% nonfat milk, the membranes were incubated overnight at 4 °C with Caspase-3 (Abcam, ab13847), Bcl-2 (Abcam, ab32124), Bcl-xl (Abcam, ab32370), PARP (Abcam, ab191217), and beta-actin (Proteintech, 66009). Apoptosis plays a key role in the pathogenesis of Parkinson disease (PD).

4668; 1:1,000; Cell Signaling Technology, Inc 1) Product Images from "Natrium Benzoate Alleviates Neuronal Apoptosis via the DJ-1-Related Anti-oxidative Stress Pathway Involving Akt Phosphorylation in a Rat Model of Traumatic Spinal Cord Injury" Search for Bioz rated products from peer reviewed research papers in life science. Anti-caspase3 Abcam ab13847 WB Anti-eIF2a Abcam ab5369 WB Anti-Sigma1 Santa Cruz Sc22948 IF Anti-CD3 (Alexa Fluor® 647-conjugated) BioLegend 100209 IF Anti-CD86 (APC-conjugated) BioLegend 105011 FC Anti-CD11b (Alexa Fluor® 488-conjugated) BioLegend 101217 FC Anti-CD11c (FITC-conjugated) BioLegend 117305 FC Anti-CD4 (FITC-conjugated) BioLegend 100405 FC 2018-08-23 · Next, some of the sections were incubated with anti-β-adrenergic receptor antibody (Cat #: ab3442, Abcam, Cambridge, MA, USA) and other sections were incubated with anti-p53 antibody (Cat #: sc-6243, Santa Cruz Biotechnology, Santa Cruz, CA, USA); the rest of the sections were incubated with anti-active caspase-3 antibody (Cat #: ab13847, Abcam, Cambridge, MA, USA) using the dilutions suggested by the manufacturer. (1: 2000; Catalogue No. ab13847, Abcam Bio-tech., Cambridge, MA, USA), rabbit anti-mouse B-cell lymphoma-2 (Bcl-2) polyclonal antibody (1: 2000, Catalogue No. ab59348, Abcam Bio-tech., Cambridge, MA, USA), rabbit anti-mouse Bcl-2 associated protein X (Bax) polyclonal an-tibody (1: 2000, Catalogue No. ab182733) and 2018-04-03 · Slides were placed on the Bond Max autostainer for staining with the Abcam antibody for Active Caspase 3 (Rabbit polyclonal, ab13847). The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/mL, applied to tissues for 15 minutes, and detected with Abcam Steady DAB/Plus (ab103723) for 5 minutes.
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Comments: validation status unknown, seller recommendations provided in 2012: Flow Cytometry; Immunocytochemistry; Immunohistochemistry; Western Blot; Flow Cytometry, The following primary antibodies were used: Anti-vimentin (cat. no.

Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE.
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Cheng et al. Benzyl Isothiocyanate INTRODUCTION Mammary gland carcinomas are the most common cancers in women and intact female dogs (1). Breast cancer is a growing

Mouse monoclonal nology, #9504), pro-caspase-3 (1:1000, Abcam, #ab13847), cleaved caspase-3 ( 1:1000, Abcam, #ab49822), PARP. (1:1000, Abcam, #ab32064), Bcl2 (1:1000, Watercolor Circles No1 Poster / Abstrakt konst hos Desenio AB (13847).

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Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST.

Introduction. Breast cancer is one of the most common malignant tumor in women worldwide with a high morbidity and mortality. It is estimated that there are 2 million new cases and 630,000 deaths from breast cancer every year, accounting for 14% of all cancer-related deaths among women worldwide (Ferlay et al., 2019).